GSE78164Expression data from CD34+/CD71- cells following knockdown of HSPA9
Homo sapiens
8 Downloadable Samples
Affymetrix Human Gene 1.0 ST Array (hugene10st)Submitter Supplied Information
Description
Knockdown of HSPA9 causes a dose-dependent decrease in erythroid maturation of CD34+ cells differentiated in culture. Due to differences in the degree of differentiation, a more homogeneous population was selected for using FACS and the gene expression profile of these cells was compared. We used a lentiviral vector (pLKO.1) expressing short hairpin RNAs targeting either luciferase (control shLUC) or HSPA9 (shHSPA9-433) to knock down expression of HSPA9.
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LUC control knockdown CD34+/CD71- cord blood derived cells, biological replicate 2
cd34+ cells
LUC control knockdown CD34+/CD71- cord blood derived cells, biological replicate 3
cd34+ cells
LUC control knockdown CD34+/CD71- cord blood derived cells, biological replicate 4
cd34+ cells
LUC control knockdown CD34+/CD71- cord blood derived cells, biological replicate 1
cd34+ cells
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